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Posts tagged ‘structural biology’

Cryo-electron microscopy: big kit solving life’s smallest puzzles

Since its invention in the 1980s, the UK has led developments in cryo-electron microscopy – a technique allowing scientists to zoom in on the inner-workings of tiny molecules. A new imaging centre at the MRC-University of Glasgow Centre for Virus Research is now expanding the UK’s powerful microscope offering. Director Professor David Bhella shares his grand plans.

So what exactly is cryo-electron microscopy?

David Bhella with imaging computer

Cryo-electron microscopy, or cryo-EM for short, is a powerful microscope technique. It’s different from imaging with normal microscopes as it uses beams of electrons, instead of light. Structural biologists use this technique to take pictures of biological samples at near-atomic scale, frozen in their natural state. It’s a faster and more effective way of looking deep inside molecules compared to other structural biology tools. Read more

Practical science: the lab technician

Pat Edwards is a Research Support Technician in the Structural Studies Division at the MRC Laboratory of Molecular Biology (LMB) in Cambridge. She spoke to The Long+Short about her job.

Pat Edwards

Pat Edwards (Image copyright: Chris Tate)

I suppose I am an archetypal technician. We have a lot of new people just trying to work out what’s going on, so I’m a knowledge base for a lot of the things, the methods and technologies, which go on in the lab.

We do structural biology of membrane proteins, which has huge implications for medicine. I do anything from expressing those proteins, to purification and crystallisation. I work with my boss and a postdoc on a project that will change depending on who that is. My work is acknowledged and I am always on the papers that result from it.

My background is an applied biology degree. I was interested in doing science, but not in doing a PhD – I’m not very good at studying, but I’m a very practical person. My first job was actually here in the LMB, and I guess I really learnt the trade, if you could call it that, in the lab – which is really the more practical side of science. Read more

Behind the picture: New microscope tech that’s as good as gold

Christmas decoration? Modern art? Anything to do with science at all? Of course it is. As well as being pretty to look at, this little grid full of holes could have a big impact on microscopy. Dr Chris Russo, a researcher at the MRC Laboratory of Molecular Biology (MRC LMB) and the person who took the photo, explains more.

A close-up of a gold grid, showing a cross-hatched pattern with internal squares of dots or holes

It might look like something you should hang on your wall, but this picture is actually a close-up of a tiny gold device that could allow researchers to unravel the details of how the complex biological machines inside cells work.

Working with Dr Lori Passmore, I have used this ‘grid’, which costs just a few pounds to make, to almost double the image quality of a multi-million pound electron microscope.

We then used it to determine the structure of a protein called ferritin, a small protein cage which stores the iron that cells need to function, and a particularly tough structure to determine. Read more